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Description
Rat B-NGF (Beta Nerve Growth Factor) ELISA KitThe Rat (B NGF) Beta Nerve Growth Factor ELISA Kit measures Beta Nerve Growth Factor in Rat samples. The plate has been pre coated with Rat NGF antibody. NGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NGF Antibody is added and binds to NGF in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NGF antibody. After incubation unbound Streptavidin HRP is washed away during a
The Rat (B-NGF) Beta Nerve Growth Factor ELISA Kit measures Beta Nerve Growth Factor in Rat samples. The plate has been pre-coated with Rat NGF antibody. NGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NGF Antibody is added and binds to NGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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